Reliability of spotlighting for tracking trends in Northern Greater Glider (Petauroides minor) numbers was assessed by repeating three 1-km transects over three consecutive nights, using the same observers and at the same times for each transect, in Blackbraes National Park, north-eastern Queensland. We found that this repeated spotlighting resulted in a method precision of about 25%, based on a coefficient of variation of 25.0. From this finding, we conclude that spotlighting is a sufficiently precise method for monitoring trends in P. minor populations under consistent and favourable environmental conditions.